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probdnf  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology probdnf
    Probdnf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+probdnf/pro+BDNF+Antibody/pm41671728-128-29-52
    Average 94 stars, based on 257 article reviews
    probdnf - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Aging-Induced Brain-Derived Neurotrophic Factor in Adipocyte Progenitors Contributes to Adipose Tissue Dysfunction
    Article Snippet: .. The following primary antibodies were used for western blot analysis: anti-proBDNF (mouse, Santa Cruz Biotechnology), anti-tyrosine hydroxylase (mouse, Merck Millipore), phosphoHSL(Ser660, rabbit, Cell Signaling), HSL (rabbit, Cell Signaling), anti-PDGFR (goat, R&D system), PLIN1 (rabbit, Abcam), anti-p21 (rabbit, Cell Signaling), anti- TNF (goat, R&D system), anti-Sortilin (goat, R&D system), anti-cleaved caspase 3(rabbit, Cell Signaling), anti-caspase 3(rabbit, Cell Signaling), anti-RIP3(rabbit, Cell Signaling), anti-phospho-RIP3(Thr231/Ser232, rabbit, Cell Signaling), anti-Na/K ATPase (rabbit, Abcam) Total OXPHOS Rodent WB Antibody Cocktail (Abcam), anti-Actin (mouse, Santa Cruz Bio- technology), and anti-/Tubulin (rabbit, Cell Signaling). ..

    Control:

    Article Title: Zeb2/Axin2-Enriched BMSC-Derived Exosomes Promote Post-Stroke Functional Recovery by Enhancing Neurogenesis and Neural Plasticity.
    Article Snippet: Exosomes harvested from bone marrow-derived mesenchymal stromal cells (BMSCs) have shown treatment potential in many diseases.. In vitro, Zeb2/Axin2 stimulated endogenous neurogenesis, which induced functional recovery after stroke.. Here, we investigated whether the Zeb2/Axin2-enriched exosomes harvested from BMSCs transfected with a Zeb2/Axin2 overexpression plasmid would enhance neurological recovery.

    Incubation:

    Article Title: Interesterified fat maternal consumption before conception programms memory and learning of adulthood offspring: How big is this deleterious repercussion?
    Article Snippet: In recent years, interesterified fat (IF) has largely replaced trans fat in industrialized food.. Studies of our research group showed that IF consumption may not be safe for central nervous system (CNS) functions.. Our current aim was to evaluate IF maternal consumption before conception on cognitive performance of adult rat offspring.

    Article Title: Tactile Stimulation on Adulthood Modifies the HPA Axis, Neurotrophic Factors, and GFAP Signaling Reverting Depression-Like Behavior in Female Rats.
    Article Snippet: Depression is a common psychiatric disease which pharmacological treatment relieves symptoms, but still far from ideal.. Tactile stimulation (TS) has shown beneficial influences in neuropsychiatric disorders, but the mechanism of action is not clear.. Here, we evaluated the TS influence when applied on adult female rats previously exposed to a reserpine-induced depression-like animal model.

    Article Title: Increasing brain palmitoylation rescues behavior and neuropathology in Huntington disease mice
    Article Snippet: .. The incubation with primary antibodies was made overnight at 4°C in blocking solution: anti-HTT (Merck-Millipore #MAB2166; 1:100), anti-APT1 (Abcam #ab91606; 1:100), anti-GM130 (Abcam #ab52649; 1:500), anti-mCherry (Institute Curie Cat #A-P-R#13; 1:200), anti-calnexin (Sigma-Aldrich #C4731; 1:500), anti-turboGFP (CliniSciences #TA150041; 1:100), anti-Sec24C (Sigma-Aldrich #HPA040196; 1:100), anti-Active Sar1-GTP (Biomol-NewEast Biosciences #NB-26916; 1:100), anti-GFP (Millipore #AB16901; 1:200), anti-proBDNF (Santa Cruz #sc-65514; 1:100), and anti-GRASP65 (Thermo Fisher Scientific #PA3-910; 1:300). .. After 2 hours of incubation at room temperature with II antibodies, the coverslips were incubated with Hoechst (Sigma-Aldrich #B2261; 1:5000) and mounted on glass slides with ProLong Diamond Antifade Mounting (Life Technologies #P36961).

    Binding Assay:

    Article Title: Tactile Stimulation on Adulthood Modifies the HPA Axis, Neurotrophic Factors, and GFAP Signaling Reverting Depression-Like Behavior in Female Rats.
    Article Snippet: Depression is a common psychiatric disease which pharmacological treatment relieves symptoms, but still far from ideal.. Tactile stimulation (TS) has shown beneficial influences in neuropsychiatric disorders, but the mechanism of action is not clear.. Here, we evaluated the TS influence when applied on adult female rats previously exposed to a reserpine-induced depression-like animal model.

    Blocking Assay:

    Article Title: Increasing brain palmitoylation rescues behavior and neuropathology in Huntington disease mice
    Article Snippet: .. The incubation with primary antibodies was made overnight at 4°C in blocking solution: anti-HTT (Merck-Millipore #MAB2166; 1:100), anti-APT1 (Abcam #ab91606; 1:100), anti-GM130 (Abcam #ab52649; 1:500), anti-mCherry (Institute Curie Cat #A-P-R#13; 1:200), anti-calnexin (Sigma-Aldrich #C4731; 1:500), anti-turboGFP (CliniSciences #TA150041; 1:100), anti-Sec24C (Sigma-Aldrich #HPA040196; 1:100), anti-Active Sar1-GTP (Biomol-NewEast Biosciences #NB-26916; 1:100), anti-GFP (Millipore #AB16901; 1:200), anti-proBDNF (Santa Cruz #sc-65514; 1:100), and anti-GRASP65 (Thermo Fisher Scientific #PA3-910; 1:300). .. After 2 hours of incubation at room temperature with II antibodies, the coverslips were incubated with Hoechst (Sigma-Aldrich #B2261; 1:5000) and mounted on glass slides with ProLong Diamond Antifade Mounting (Life Technologies #P36961).



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    SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. <t>C-H.</t> <t>HEK293T</t> cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the <t>proBDNF</t> form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results
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    SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. <t>C-H.</t> <t>HEK293T</t> cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the <t>proBDNF</t> form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results
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    SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. <t>C-H.</t> <t>HEK293T</t> cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the <t>proBDNF</t> form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results
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    Image Search Results


    SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. C-H. HEK293T cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the proBDNF form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Long non-coding SINEUP RNA enhancing BDNF translation prevents dendritic atrophy following an Aβ-peptide challenge

    doi: 10.1007/s00018-026-06175-4

    Figure Lengend Snippet: SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. C-H. HEK293T cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the proBDNF form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results

    Article Snippet: The membrane was blocked by 5% non-fat milk in TBST- solution (1 h, RT) and then, the HEK293T cells were incubated with the rabbit polyclonal anti proBDNF antibody (Alomone, Cat. # ANT-006, dilution 1:1000, over-night at 4 °C) while the SH-SY5Y cells were incubated also with the mouse monoclonal anti-BDNF recognizing both BDNF forms (Sigma Cat. #B5050, dilution 1:1000, over-night at 4 °C). and mouse anti alpha tubulin (Sigma, Cat. # T6074, dilution 1:3000) over-night at 4 °C.

    Techniques: Alternative Splicing, Sequencing, Transfection, Control, Western Blot, Molecular Weight, Plasmid Preparation, Produced